Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: Characterization of global transcriptome in Corynebacterium glutamicum ATCC21300 and ATCC13032. ( A ) The total number of mRNA-Seq (sequencing) reads mapped in each C. glutamicum strain library; and ( B ) differentially expressed genes (DEGs) in C. glutamicum ATCC21300. Detailed information is shown in . CDS: coding sequencing.

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: Functional categorization of up- and downregulated genes in Corynebacterium glutamicum ATCC21300 based on gene ontology (GO) annotations. Detailed information is shown in .

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Functional Assay

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: Expression patterns of genes involved in biotin metabolism in Corynebacterium glutamicum ATCC13032 and ATCC21300.

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: The expression patterns of genes associated with biotin synthesis and transport in Corynebacterium glutamicum ATCC13032 and ATCC21300. ( A ) The expression pattern of bioB ; ( B ) the expression pattern of bioA ; ( C ) the expression pattern of bioD ; ( D ) the expression pattern of the bioY homolog; ( E ) the expression pattern of the bioM homolog; and ( F ) the expression pattern of the ABC transporter permease. The gene expression level (arbitrary units) was normalized using the 16s RNA level as an internal reference. Gene expression levels were quantified by real-time RT-PCR.

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Expressing, Gene Expression, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: l -Lysine production of Corynebacterium glutamicum ATCC13032, ATCC21300, and the bioB mutant (of ATCC21300). Values shown are averages based on results obtained from at least three independent experiments, in which the standard deviation was <5%.

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Mutagenesis, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: Growth rate of Corynebacterium glutamicum ATCC13032, ATCC21300, and the bioB mutant of ATCC21300.

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Mutagenesis

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome and Gene Ontology (GO) Enrichment Analysis Reveals Genes Involved in Biotin Metabolism That Affect l -Lysine Production in Corynebacterium glutamicum

doi: 10.3390/ijms17030353

Figure Lengend Snippet: The expression patterns of genes associated with biotin transport in Corynebacterium glutamicum ATCC13032, ATCC21300, and the bioB mutant (of ATCC21300). ( A ) The expression pattern of the bioY homolog; ( B ) the expression pattern of the bioM homolog; ( C ) the expression pattern of the ABC transporter permease. The gene expression level (arbitrary units) was normalized using the 16s RNA level as an internal reference. Gene expression levels were quantified by real-time RT-PCR.

Article Snippet: Kyowa Hakko reported a batch process using the C . glutamicum strain ATCC21300 (auxotrophic for threonine and leucine) that resulted in the production of 53.2 g·L −1 of l -lysine-HCl, which represented a 29% conversion yield [ ].

Techniques: Expressing, Mutagenesis, Gene Expression, Quantitative RT-PCR